Journal: bioRxiv

Article Title: Phage display profiling of CDR3β loops enables machine learning predictions of NY-ESO-1 specific TCRs

doi: 10.1101/2024.06.27.600973

Figure Lengend Snippet: (A) Heatmap showing the fraction of CD69+PD-1+ Jurkat cells encoding four TCRs with different CDR3β sequences based on the 1G4 template after co-culture with T2 cells pulsed with the NY-ESO-1 epitope at physiologically relevant peptide concentration (0.1 μg/mL). As negative control, the results for the CMV-derived epitope NLVPMVATV are also shown. For clarity, only the sequence of the core region of the CDR3β loop is shown. (B) Heatmap showing the fraction of CD69+PD-1+ Jurkat cells encoding the four TCRs of panel A after co-culture with T2 cells pulsed with three peptides from the self proteome at peptide concentration 0.1 μg/mL. The NY-ESO-1 peptide was used as positive control, and the CMV-derived epitope NLVPMVATV as negative control. (C) Fraction of CD69+PD-1+ Jurkat cells expressing the four TCRs of panel A and activated by co-culturing with HLA-A*02:01 positive Jurkat cells presenting peptides derived from the self-proteome. Jurkat cells without any TCR expression (no transduction) were used as negative controls. Jurkat cells expressing the high-affinity TCR 1G4-c53c50 were used as positive control. (D) Heatmap showing the fraction of CD69+PD-1+ Jurkat cells encoding four TCRs based on the affinity-enhanced 1G4-c50 and 1G4-c53c50 templates when stimulated by the indicated peptide at peptide concentration 0.1 μg/mL. (E) Fraction of activated Jurkat cells encoding four CDR3β sequences based on the affinity-enhanced 1G4-c50 and 1G4-c53c50 templates when stimulated by peptides derived from the self-proteome.

Article Snippet: I. Edes and W. Uckert, Max-Delbrück-Center, Berlin, Germany), TCR knock-out HLA-A2pos/J76 CD8αβ cells and HLA-A2pos TAP-deficient T2 cells (ATCC CRL-1992) were cultured at 37°C and 5% CO2 in RPMI 1640 supplemented with 10% FCS, 10 mM HEPES, 100 U/mL penicillin, 100 μg/mL streptomycin, 1X non-essential amino acids and 1mM sodium pyruvate.

Techniques: Co-Culture Assay, Concentration Assay, Negative Control, Derivative Assay, Sequencing, Positive Control, Expressing, Transduction

Journal: bioRxiv

Article Title: Phage display profiling of CDR3β loops enables machine learning predictions of NY-ESO-1 specific TCRs

doi: 10.1101/2024.06.27.600973

Figure Lengend Snippet: Heatmaps showing the fraction of CD69+PD-1+ Jurkat cells encoding four TCRs with different CDR3β sequences based on the three template TCRs. Jurkat cells were co-cultured overnight with peptide-pulsed T2 cells, at multiple peptide concentrations (0.01 μg/mL, 0.1 μg/mL, and 1 μg/mL).

Article Snippet: I. Edes and W. Uckert, Max-Delbrück-Center, Berlin, Germany), TCR knock-out HLA-A2pos/J76 CD8αβ cells and HLA-A2pos TAP-deficient T2 cells (ATCC CRL-1992) were cultured at 37°C and 5% CO2 in RPMI 1640 supplemented with 10% FCS, 10 mM HEPES, 100 U/mL penicillin, 100 μg/mL streptomycin, 1X non-essential amino acids and 1mM sodium pyruvate.

Techniques: Cell Culture

Journal: NPJ Science of Food

Article Title: A multiplex microarray lateral flow immunoassay device for simultaneous determination of five mycotoxins in rice

doi: 10.1038/s41538-024-00342-2

Figure Lengend Snippet: Comparison between the performance of microarray lateral flow device (µLFIA) and liquid chromatography–tandem mass spectrometry (LC–MS/MS) in terms of recovery, repeatability, and reproducibility for aflatoxin B 1 (AFB 1 ), T-2 toxin (T2), zearalenone (ZEA), deoxynivalenol (DON), and fumonisin B 1 (FB 1 ) at final concentration of 10 (L1), 20 (L2), and 50 µg/kg (L3)

Article Snippet: Mycotoxin standards—aflatoxin B 1 (AFB 1 ), aflatoxin B 2 (AFB 2 ), aflatoxin G 1 (AFG 1 ), aflatoxin G 2 (AFG 2 ), aflatoxin M 1 (AFM 1 ), ochratoxin A (OTA), zearalenone (ZEA), T-2 toxin (T2), HT-2 toxin (HT-2), deoxynivalenol (DON), and fumonisin B 1 (FB 1 ) were purchased from Romer Labs (Biopure, Singapore).

Techniques: Comparison, Microarray, Chromatography, Mass Spectrometry, Concentration Assay, Intra Assay, Inter Assay